From Roots to Leaves: The Capacity of <i>Micromonospora</i> to Colonize Different Legume Tissues

An important number of Micromonospora strains have been reported from nitrogen-fixing root nodules legume and actinorhizal plants. However, the question whether this bacterium can also be found in other parts these plants remains unanswered. More than 150 were recovered different Lupinus angustifolius Pisum sativum tissues, including leaves, stems, roots, nodules. In all, 97% isolates identified by 16S ribosomal RNA gene sequence target genus associated with 27 spp. Plant-polymer-degrading enzymes are suspected to play a role colonization To end, bacterial enzymatic activity assays for amylases, cellulases, chitinases, pectinases, xylanases determined. All produced while 98.6, 98, 94.6% them respectively. The most productive included seven P. one L. angustifolius. Strain lupini ML01-gfp was used determine its capacity reach colonize plant organs using as model. Stem leaf samples monitored optical fluorescence microscopy locate tagged strain. These results strongly suggest that is able not only infect but host plant, especially leaves.